8VUC

Structure of FabS1CE2-EPR-1, an elbow-locked high affinity antibody for the erythropoeitin receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979180
Spacegroup name	P 32
Unit cell lengths	67.389, 67.389, 168.232
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	55.140 - 2.500
R-factor	0.2081
Rwork	0.204
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.056
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.120	2.550
High resolution limit [Å]	2.450	2.450
Rmerge	0.107	1.135
Rmeas	0.121	1.291
Rpim	0.057	0.609
Number of reflections	29855	3577
<I/σ(I)>	7.5	1.4
Completeness [%]	94.9	99.9
Redundancy	4.3	4.4
CC(1/2)	0.993	0.417

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	298	0.1 M MMT buffer pH 5.0, 25% PEG1500