8VUC
Structure of FabS1CE2-EPR-1, an elbow-locked high affinity antibody for the erythropoeitin receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979180 |
Spacegroup name | P 32 |
Unit cell lengths | 67.389, 67.389, 168.232 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 55.140 - 2.500 |
R-factor | 0.2081 |
Rwork | 0.204 |
R-free | 0.26530 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.056 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.120 | 2.550 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.107 | 1.135 |
Rmeas | 0.121 | 1.291 |
Rpim | 0.057 | 0.609 |
Number of reflections | 29855 | 3577 |
<I/σ(I)> | 7.5 | 1.4 |
Completeness [%] | 94.9 | 99.9 |
Redundancy | 4.3 | 4.4 |
CC(1/2) | 0.993 | 0.417 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 0.1 M MMT buffer pH 5.0, 25% PEG1500 |