8VUA

Structure of FabS1CE1-EPR-1, an elbow-locked high affinity antibody for the erythropoeitin receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 31 2 1
Unit cell lengths	72.933, 72.933, 153.366
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.400 - 3.270
R-factor	0.2472
Rwork	0.245
R-free	0.28230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.609
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	153.370	3.350
High resolution limit [Å]	3.130	3.130
Rmerge	0.074	2.017
Rmeas	0.078	2.121
Rpim	0.025	0.651
Number of reflections	8839	1569
<I/σ(I)>	19.5	1.2
Completeness [%]	99.9	100
Redundancy	9.8	10.5
CC(1/2)	1.000	0.505

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1 M HEPES 7.0, 30% Jeffamine ED-2003