8VU4

Structure of FabS1CE4-EPR-1, an elbow-locked high affinity antibody for the erythropoeitin receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1
Unit cell lengths	55.863, 55.893, 87.016
Unit cell angles	80.05, 89.91, 82.70

Refinement procedure

Resolution	55.400 - 2.350
R-factor	0.2105
Rwork	0.208
R-free	0.25160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.063
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.690	2.340
High resolution limit [Å]	2.270	2.270
Rmerge	0.115	0.479
Rmeas	0.162	0.677
Rpim	0.115	0.479
Number of reflections	43559	4024
<I/σ(I)>	8.3	1.7
Completeness [%]	91.5	91.6
Redundancy	2	1.9
CC(1/2)	0.965	0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	298	0.1 M BICINE 9.0, 10% PEG6000