8VU4
Structure of FabS1CE4-EPR-1, an elbow-locked high affinity antibody for the erythropoeitin receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.863, 55.893, 87.016 |
| Unit cell angles | 80.05, 89.91, 82.70 |
Refinement procedure
| Resolution | 55.400 - 2.350 |
| R-factor | 0.2105 |
| Rwork | 0.208 |
| R-free | 0.25160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.063 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.690 | 2.340 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.115 | 0.479 |
| Rmeas | 0.162 | 0.677 |
| Rpim | 0.115 | 0.479 |
| Number of reflections | 43559 | 4024 |
| <I/σ(I)> | 8.3 | 1.7 |
| Completeness [%] | 91.5 | 91.6 |
| Redundancy | 2 | 1.9 |
| CC(1/2) | 0.965 | 0.508 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 0.1 M BICINE 9.0, 10% PEG6000 |
