8VU1

Structure of FabS1CE3-EPR-1, an elbow-locked high affinity antibody for the erythropoeitin receptor (trigonal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 31 2 1
Unit cell lengths	73.608, 73.608, 153.886
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	63.750 - 3.080
R-factor	0.2375
Rwork	0.235
R-free	0.27670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.633
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	153.890	3.110
High resolution limit [Å]	2.930	2.930
Rmerge	0.075	2.384
Rmeas	0.079	2.507
Rpim	0.025	0.767
Number of reflections	10941	1727
<I/σ(I)>	19.9	1
Completeness [%]	100.0	100
Redundancy	10	10.6
CC(1/2)	1.000	0.496

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	298	0.1 M PCPT buffer pH 9.0, 25% PEG1500