8VU0

Co-crystal structure of Aquifex aeolicus Trbp111 in complex with E. coli tRNA-Ile

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	190.495, 55.028, 87.160
Unit cell angles	90.00, 94.42, 90.00

Refinement procedure

Resolution	43.090 - 2.640
R-factor	0.1845
Rwork	0.182
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.739
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.090	2.730
High resolution limit [Å]	2.640	2.640
Rpim	0.044	0.197
Number of reflections	26578	2645
<I/σ(I)>	9.76	0.87
Completeness [%]	98.8
Redundancy	12.6
CC(1/2)	0.977	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294.15	0.2 M ammonium phosphate dibasic, 23% PEG 3350