8VTY
Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with ciprofloxacin and protein Y at 2.60A resolution
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-24 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97911 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.900, 449.760, 620.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 122.000 - 2.600 |
R-factor | 0.2053 |
Rwork | 0.203 |
R-free | 0.24130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xhx |
RMSD bond length | 0.003 |
RMSD bond angle | 0.647 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 122.000 | 122.000 | 2.670 |
High resolution limit [Å] | 2.600 | 11.630 | 2.600 |
Rmerge | 0.249 | 0.058 | 2.757 |
Rmeas | 0.266 | 0.063 | 2.949 |
Total number of observations | 13697385 | ||
Number of reflections | 1772270 | 20606 | 130169 |
<I/σ(I)> | 7.9 | 26.82 | 0.88 |
Completeness [%] | 99.9 | 98.4 | 99.6 |
Redundancy | 7.729 | 7.653 | 7.767 |
CC(1/2) | 0.991 | 0.995 | 0.161 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |