8VTT
Meis1 homeobox domain bound to neomycin fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97976 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 77.757, 77.757, 213.298 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.880 - 2.450 |
R-factor | 0.2184 |
Rwork | 0.215 |
R-free | 0.25690 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.004 |
RMSD bond angle | 0.424 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
High resolution limit [Å] | 2.450 | 6.650 | 2.450 |
Rmerge | 0.181 | 0.073 | 1.277 |
Rmeas | 0.188 | 0.076 | 1.400 |
Rpim | 0.052 | 0.021 | 0.556 |
Total number of observations | 330178 | ||
Number of reflections | 27646 | 1471 | 1360 |
<I/σ(I)> | 5.2 | ||
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 11.9 | 13.2 | 6.2 |
CC(1/2) | 0.997 | 0.996 | 0.479 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 2.60 M ammonium sulfate, 0.15 M sodium chloride, 20 mM Tris, 4% 2-propanol, 10 mM neomycin, 25% ethylene glycol |