8VTT

Meis1 homeobox domain bound to neomycin fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97976
Spacegroup name	P 32 1 2
Unit cell lengths	77.757, 77.757, 213.298
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.880 - 2.450
R-factor	0.2184
Rwork	0.215
R-free	0.25690
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.004
RMSD bond angle	0.424
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.450	6.650	2.450
Rmerge	0.181	0.073	1.277
Rmeas	0.188	0.076	1.400
Rpim	0.052	0.021	0.556
Total number of observations	330178
Number of reflections	27646	1471	1360
<I/σ(I)>	5.2
Completeness [%]	100.0	99.6	100
Redundancy	11.9	13.2	6.2
CC(1/2)	0.997	0.996	0.479

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	2.60 M ammonium sulfate, 0.15 M sodium chloride, 20 mM Tris, 4% 2-propanol, 10 mM neomycin, 25% ethylene glycol