8VTS
Meis1 homeobox domain bound to paromomycin fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97976 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 77.621, 77.621, 213.724 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.830 - 1.910 |
| R-factor | 0.1916 |
| Rwork | 0.191 |
| R-free | 0.21090 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.489 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.079 | 0.037 | 1.808 |
| Rmeas | 0.082 | 0.038 | 1.899 |
| Rpim | 0.023 | 0.010 | 0.573 |
| Total number of observations | 717867 | ||
| Number of reflections | 57573 | 3025 | 2897 |
| <I/σ(I)> | 6.8 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 12.5 | 13.4 | 10.7 |
| CC(1/2) | 0.997 | 0.999 | 0.532 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 2.20 M ammonium sulfate, 0.15 M sodium chloride, 20 mM Tris, 4% 2-propanol, 10 mM paromomycin, 25% ethylene glycol |
