8VTR

Structure of FabS1CE3-EPR-1, an elbow-locked high affinity antibody for the erythropoeitin receptor (orthorhombic form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	73.199, 74.275, 93.335
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.120 - 1.960
R-factor	0.1893
Rwork	0.187
R-free	0.22750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.976
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.280	2.010
High resolution limit [Å]	1.960	1.960
Rmerge	0.073	0.686
Rmeas	0.079
Rpim	0.031	0.302
Number of reflections	37275	2602
<I/σ(I)>	18.5	2.3
Completeness [%]	99.9	99.7
Redundancy	6.5	6
CC(1/2)	0.999	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M Ammonium Citrate dibasic, 20% PEG3350. This was set up as a Fab/EPOR complex, however no EPOR was observed upon solving the structure.