8VTP

Structure of FabS1CE-EPR-1, a high affinity antibody for the erythropoeitin receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-10
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	66.750, 72.938, 129.581
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.790 - 1.770
R-factor	0.1757
Rwork	0.175
R-free	0.19500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.952
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	129.580	1.710
High resolution limit [Å]	1.680	1.680
Rmerge	0.061	1.902
Rmeas	0.064	2.041
Rpim	0.020	0.723
Number of reflections	72770	3605
<I/σ(I)>	19.3	0.8
Completeness [%]	99.9	98
Redundancy	9.6	7.6
CC(1/2)	0.999	0.352

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	0.1 M Ammonium Acetate, 0.1 M Bis Tris pH 5.5, 17% PEG10K