8VTN
Crystal structure of R. sphaeroides Photosynthetic Reaction Center variant Y(M210)2-nitrophenylalanine
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 250 |
Detector technology | PIXEL |
Collection date | 2023-08-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97893 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 141.490, 141.490, 187.510 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.450 - 3.570 |
R-factor | 0.2127 |
Rwork | 0.211 |
R-free | 0.24650 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.741 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.450 | 3.698 |
High resolution limit [Å] | 3.570 | 3.570 |
Rmerge | 0.312 | 1.409 |
Rmeas | 0.328 | 1.543 |
Rpim | 0.115 | 0.508 |
Number of reflections | 26248 | 2593 |
<I/σ(I)> | 6.66 | 1.46 |
Completeness [%] | 99.5 | 99.92 |
Redundancy | 7.8 | 8 |
CC(1/2) | 0.994 | 0.659 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1 M potassium phosphate, 3.5% 1,2,3-heptanetriol, and 0.1% LDAO precipitant solution; 1.4-1.5 M potassium phosphate reservoir solution, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |