8VTK
Crystal structure of R.sphaeroides Photosynthetic Reaction Center variant Y(M210)2-chlorophenylalanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 250 |
Detector technology | PIXEL |
Collection date | 2023-08-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97893 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 140.950, 140.950, 186.720 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.760 - 3.070 |
R-factor | 0.1789 |
Rwork | 0.177 |
R-free | 0.21270 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.385 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.760 | 3.180 |
High resolution limit [Å] | 3.070 | 3.070 |
Rmerge | 0.264 | 2.075 |
Rmeas | 0.269 | 2.238 |
Rpim | 0.066 | 0.507 |
Number of reflections | 40589 | 3968 |
<I/σ(I)> | 12.68 | 1.48 |
Completeness [%] | 99.9 | 99.97 |
Redundancy | 16.8 | 17.7 |
CC(1/2) | 0.998 | 0.621 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1 M potassium phosphate, 3.5% 1,2,3-heptanetriol, and 0.1% LDAO precipitant solution; 1.4-1.5 M potassium phosphate reservoir solution, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |