8VST
Rns mutant H20A/R75A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979351 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.650, 97.290, 136.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.650 - 2.400 |
| R-factor | 0.2488 |
| Rwork | 0.245 |
| R-free | 0.29640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.258 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.650 | 2.486 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 25620 | 2499 |
| <I/σ(I)> | 6.49 | 0.78 |
| Completeness [%] | 99.7 | 99.84 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.995 | 0.383 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M succinnic acid, 14% PEG 3350, 0.03 M glycyl-glycyl-gylcine, 50% v/v DMSO |
