8VSL

Binary structure of 14-3-3 sigma and ARAF phosphopeptide (pS214)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.967697
Spacegroup name	C 2 2 21
Unit cell lengths	82.518, 112.806, 62.713
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.600 - 1.420
R-factor	0.1836
Rwork	0.182
R-free	0.20798
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.807
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PDB-REDO

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.600	1.440
High resolution limit [Å]	1.420	1.420
Number of reflections	55423	2702
<I/σ(I)>	11.8	1.5
Completeness [%]	99.9
Redundancy	13.4
CC(1/2)	0.997	0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	PEG400, HEPES, CaCl2, glycerol