Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.18053 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.787, 44.071, 86.592 |
| Unit cell angles | 90.00, 111.07, 90.00 |
Refinement procedure
| Resolution | 40.400 - 2.040 |
| R-factor | 0.1353 |
| Rwork | 0.133 |
| R-free | 0.17190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.606 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.420 | 2.117 |
| High resolution limit [Å] | 2.040 | 2.044 |
| Rmerge | 0.033 | 0.084 |
| Number of reflections | 37834 | 3714 |
| <I/σ(I)> | 43.41 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.6 | |
| CC(1/2) | 1.000 | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | 20% PEG 3350, 0.2 M MgCl2, 1 X PBS, 20% Glycerol |
