Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-14 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.466, 90.558, 91.335 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.670 - 1.340 |
| R-factor | 0.1397 |
| Rwork | 0.139 |
| R-free | 0.16050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.979 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.950 | 1.370 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.075 | 1.387 |
| Rmeas | 0.080 | 1.473 |
| Rpim | 0.027 | 0.488 |
| Total number of observations | 1245456 | 55759 |
| Number of reflections | 139493 | 6460 |
| <I/σ(I)> | 10.1 | 0.9 |
| Completeness [%] | 99.7 | |
| Redundancy | 8.9 | 8.6 |
| CC(1/2) | 0.999 | 0.609 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.2 M Magnesium formate dihydrate 20% w/v PEG 3350 |
