8VRQ
Rns pocket mutant - R75A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97864 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.730, 96.970, 136.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.220 - 2.400 |
| R-factor | 0.2428 |
| Rwork | 0.239 |
| R-free | 0.28590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.175 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.220 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 25501 | 2497 |
| <I/σ(I)> | 7.03 | |
| Completeness [%] | 99.5 | 99.64 |
| Redundancy | 6.4 | |
| CC(1/2) | 0.996 | 0.395 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | 0.03 M glycyl-glycyl-gylcine, 14% PEG 3350, 0.1 M succinic acid, 30% DMSO |
