8VQR
Crystal structure of chimeric SARS-CoV-2 RBD complexed with chimeric raccoon dog ACE2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-10-16 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.961, 118.107, 112.233 |
Unit cell angles | 90.00, 92.89, 90.00 |
Refinement procedure
Resolution | 58.410 - 2.565 |
R-factor | 0.2165 |
Rwork | 0.214 |
R-free | 0.26420 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond angle | 13.051 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 112.091 | 2.838 |
High resolution limit [Å] | 2.565 | 2.565 |
Rmerge | 0.049 | 0.733 |
Rmeas | 0.057 | 0.847 |
Rpim | 0.028 | 0.420 |
Total number of observations | 166083 | 8531 |
Number of reflections | 42428 | 2121 |
<I/σ(I)> | 9.8 | 1.6 |
Completeness [%] | 62.8 | |
Redundancy | 3.9 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100 mM Tris (pH 8-8.5), 18-22% PEG 6000, 100 mM NaCl and ethylene glycol (0.5-2%) |