8VQK

YcjN from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 1
Unit cell lengths	63.216, 63.290, 74.314
Unit cell angles	110.01, 91.09, 118.66

Refinement procedure

Resolution	35.650 - 1.950
R-factor	0.2177
Rwork	0.215
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.805
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.400	2.060
High resolution limit [Å]	1.950	1.950
Rpim	0.088
Number of reflections	61761	6203
<I/σ(I)>	3.9
Completeness [%]	91.4
Redundancy	3.9
CC(1/2)		0.293

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.01 M CdCl2, 0.2 M NH4SO4, 0.1 M HEPES pH 7.5, and 25 % PSM