8VPU
Crystal structure of mouse Importin alpha in complex with dengue virus 3 NS5 central NLS peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.951 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.920, 89.440, 100.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.340 - 2.100 |
R-factor | 0.188 |
Rwork | 0.187 |
R-free | 0.20770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.681 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.340 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rpim | 0.051 | 0.422 |
Number of reflections | 40704 | 3382 |
<I/σ(I)> | 11 | |
Completeness [%] | 97.6 | |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 0.75 sodium citrate, 0.1 M HEPES |