8VON

Double phenylalanine Apex domain mutant of bacteriophage P2 central spike protein, membrane-piercing module

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95370
Spacegroup name	P 1
Unit cell lengths	45.570, 49.510, 89.540
Unit cell angles	106.49, 89.74, 112.34

Refinement procedure

Resolution	34.770 - 1.150
R-factor	0.1145
Rwork	0.114
R-free	0.12760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.098
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.180
High resolution limit [Å]	1.150	1.150
Rmeas	0.076	0.267
Number of reflections	489332	34586
<I/σ(I)>	13.62	5.55
Completeness [%]	98.7	94
Redundancy	4.81	4.33
CC(1/2)	0.992	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	26-33% Pentaerythritol ethoxylate (15/4 EO/OH) aka PEE-797, 100 mM Bis-Tris or MES pH 6.0-6.5