8VO1

Pathogenesis related 10-10 C155S mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	48.400, 70.836, 92.205
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.670 - 1.800
R-factor	0.2064
Rwork	0.203
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.018
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.700	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	15041	1088
<I/σ(I)>	29.15	1.9
Completeness [%]	99.7
Redundancy	10.9
CC(1/2)	1.000	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	23% polyethylene glycol 3350, 0.1M Bis-Tris pH 5.5, 0.2M sodium chloride