8VNQ
Homing endonuclease H98A I-PpoI-DNA complex at pH6.0 (K+ MES) with 1 mM Mn2+ for 1800s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-05-26 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.9765 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 114.646, 114.646, 89.057 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.200 - 1.930 |
R-factor | 0.1941 |
Rwork | 0.193 |
R-free | 0.21020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.132 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.200 | 48.200 | 2.050 |
High resolution limit [Å] | 1.930 | 5.750 | 1.930 |
Rmerge | 0.198 | 0.103 | 0.709 |
Rmeas | 0.221 | 0.113 | 0.790 |
Number of reflections | 97783 | 3722 | 15294 |
<I/σ(I)> | 4.8 | ||
Completeness [%] | 99.3 | ||
Redundancy | 9.8 | ||
CC(1/2) | 0.981 | 0.992 | 0.757 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.2 M Sodium Malonate, PEG 3350, 0.1 M MES |