8VNO
Homing endonuclease I-PpoI-DNA complex:reaction at pH6.0 (K+ MES) with 500 uM Mn2+ for 600s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2023-03-28 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9765 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 114.060, 114.060, 88.020 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.860 - 1.700 |
R-factor | 0.1794 |
Rwork | 0.179 |
R-free | 0.19320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.206 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.860 | 47.860 | 1.800 |
High resolution limit [Å] | 1.700 | 5.070 | 1.700 |
Rmerge | 0.077 | 0.032 | 0.877 |
Rmeas | 0.085 | 0.035 | 0.968 |
Number of reflections | 142915 | 5396 | 22937 |
<I/σ(I)> | 13.47 | ||
Completeness [%] | 99.8 | ||
Redundancy | 11.1 | ||
CC(1/2) | 0.999 | 0.999 | 0.725 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.2 M Sodium Malonate, PEG 3350, 0.1 M MES |