8VNH
Homing endonuclease I-PpoI-DNA complex:reaction at pH6.0 (K+ MES) with 500 uM Mn2+ for 80s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2023-03-28 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9765 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 114.060, 114.060, 88.020 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.390 - 1.760 |
R-factor | 0.1821 |
Rwork | 0.181 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.129 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.390 | 49.390 | 1.860 |
High resolution limit [Å] | 1.760 | 5.240 | 1.760 |
Rmerge | 0.099 | 0.056 | 0.871 |
Rmeas | 0.110 | 0.062 | 0.961 |
Number of reflections | 126673 | 4777 | 20414 |
<I/σ(I)> | 9.73 | ||
Completeness [%] | 99.9 | ||
Redundancy | 11 | ||
CC(1/2) | 0.997 | 0.996 | 0.740 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.2 M Sodium Malonate, PEG 3350, 0.1 M MES |