8VMW
Homing endonuclease I-PpoI-DNA complex:ground state at pH6.0 (K+ MES) with Na+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-05-26 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.97648 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 113.880, 113.880, 88.220 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.310 - 1.600 |
R-factor | 0.1847 |
Rwork | 0.184 |
R-free | 0.19840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.111 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.310 | 49.310 | 1.690 |
High resolution limit [Å] | 1.600 | 4.770 | 1.600 |
Rmerge | 0.063 | 0.028 | 0.707 |
Rmeas | 0.070 | 0.031 | 0.793 |
Number of reflections | 168718 | 6322 | 27255 |
<I/σ(I)> | 15.46 | ||
Completeness [%] | 99.7 | ||
Redundancy | 9.9 | ||
CC(1/2) | 0.999 | 0.999 | 0.764 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.2 M Sodium Malonate, PEG 3350, 0.1 M MES |