8VMR
Homing endonuclease I-PpoI-DNA complex:reaction at pH7.0 (K+ MES) with 500 uM Mg2+ for 40s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2023-03-14 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9787 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 113.580, 113.580, 87.990 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.930 - 1.500 |
R-factor | 0.1833 |
Rwork | 0.183 |
R-free | 0.19390 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.085 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.930 | 42.930 | 1.590 |
High resolution limit [Å] | 1.500 | 4.480 | 1.500 |
Rmerge | 0.084 | 0.047 | 0.903 |
Rmeas | 0.093 | 0.051 | 1.001 |
Number of reflections | 203970 | 7664 | 32605 |
<I/σ(I)> | 10.24 | ||
Completeness [%] | 99.8 | ||
Redundancy | 10.9 | ||
CC(1/2) | 0.998 | 0.998 | 0.725 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Sodium Malonate, PEG 3350, 0.1 M MES |