8VM9
The crystal structure of rhinovirus B14 RNA replication element sD-loop mutant in complex with Fab BL3-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.921, 79.214, 141.324 |
Unit cell angles | 90.00, 98.87, 90.00 |
Refinement procedure
Resolution | 69.820 - 2.200 |
R-factor | 0.2011 |
Rwork | 0.200 |
R-free | 0.22940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.019 |
Data reduction software | RAPD |
Data scaling software | RAPD |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 139.630 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.069 | |
Number of reflections | 77582 | 4411 |
<I/σ(I)> | 11.2 | |
Completeness [%] | 98.3 | |
Redundancy | 3.5 | |
CC(1/2) | 0.997 | 0.662 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 0.1 M ammonium sulfate, 0.01 M magnesium chloride hexahydrate, 0.05 M MES monohydrate pH 5.6, 20% w/v polyethylene glycol 8000. |