8VL8
Salmonella enterica Typhimurium taxis to serine and repellents (Tsr) ligand-binding domain with L-Ser, pH 7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-05-23 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9774 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 126.022, 74.771, 128.931 |
Unit cell angles | 90.00, 116.14, 90.00 |
Refinement procedure
Resolution | 61.500 - 2.500 |
R-factor | 0.2275 |
Rwork | 0.226 |
R-free | 0.27340 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.214 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.380 | 2.180 |
High resolution limit [Å] | 2.120 | 2.120 |
Number of reflections | 72723 | 4009 |
<I/σ(I)> | 5.4 | |
Completeness [%] | 98.3 | 88.5 |
Redundancy | 6.1 | 4.7 |
CC(1/2) | 0.977 | 0.568 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.5 ul protein at 7 mg per ml in 50 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.5 ul of 6 mM L-serine, and 1.5 ul 1.62 M potassium phosphate pH 7 |