8VK5
Human Sputum Leucocyte Elastase (uncomplexed)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 63 |
| Unit cell lengths | 73.570, 73.570, 70.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.230 - 1.560 |
| R-factor | 0.1417 |
| Rwork | 0.141 |
| R-free | 0.15860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.151 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.230 | 1.616 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Number of reflections | 40278 | 3078 |
| <I/σ(I)> | 12.67 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.9 | 13.1 |
| CC(1/2) | 0.991 | 0.971 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 296 | 0.2 M Lithium sulfate monohydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000 |
