8VJY
Structure of Human Neurolysin in complex with Neurotensin peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.712, 150.576, 188.799 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.510 - 1.950 |
| R-factor | 0.1994 |
| Rwork | 0.198 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 14.108 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.510 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.166 | 2.914 |
| Rmeas | 0.181 | 3.286 |
| Rpim | 0.071 | 1.471 |
| Total number of observations | 783426 | 21292 |
| Number of reflections | 123468 | 4926 |
| <I/σ(I)> | 9.3 | 0.5 |
| Completeness [%] | 99.0 | |
| Redundancy | 6.3 | 4.3 |
| CC(1/2) | 0.995 | 0.157 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 17.5 ~ 30 % polyethylene glycol 3,350 and 50 ~ 125 mM Bis-Tris HCl buffer, pH 6.5 |
