8VJN

Myxococcus xanthus encapsulin cargo protein EncD in complex with flavin mononucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9774
Spacegroup name	C 1 2 1
Unit cell lengths	87.566, 27.999, 86.469
Unit cell angles	90.00, 96.83, 90.00

Refinement procedure

Resolution	32.540 - 2.310
R-factor	0.1879
Rwork	0.183
R-free	0.23060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.052
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2-3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.930	2.400
High resolution limit [Å]	2.310	2.310
Rmerge	0.058	0.840
Number of reflections	9203	726
<I/σ(I)>	8.93
Completeness [%]	96.9	80
Redundancy	2	2
CC(1/2)	0.997	0.370

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	289.15	25% PEG 1500, 0.1 M PCTP (sodium propionate, sodium cacodylate and Bis-Tris propane, 2:1:2)