Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-03 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.107, 69.107, 79.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.710 - 1.700 |
| R-factor | 0.1999 |
| Rwork | 0.199 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.016 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.710 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.048 | 1.026 |
| Number of reflections | 24551 | 1304 |
| <I/σ(I)> | 30.7 | 2.2 |
| Completeness [%] | 100.0 | |
| Redundancy | 9.7 | |
| CC(1/2) | 1.000 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | HEPES-NaOH, BisTris, PEG400, TCEP |
