8VJ5

Peroxide-Soaked MnSOD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-03-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 61 2 2
Unit cell lengths	78.348, 78.348, 236.628
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.100 - 1.760
R-factor	0.1985
Rwork	0.198
R-free	0.21770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.572
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.760	1.760
Rpim	0.104	0.278
Number of reflections	38708	2947
<I/σ(I)>	5.96
Completeness [%]	88.4
Redundancy	6.1
CC(1/2)	0.872	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	1.8 M Potassium Phosphate