8VJ1

Structure of C-terminal domain of telomere resolvase, ResT, from Borrelia garinii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 43 3 2
Unit cell lengths	114.583, 114.583, 114.583
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.510 - 2.260
R-factor	0.1935
Rwork	0.188
R-free	0.24650
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.854
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.290
High resolution limit [Å]	2.250	2.250
Number of reflections	12747	628
<I/σ(I)>	28.7
Completeness [%]	99.9
Redundancy	104.8
CC(1/2)	0.902	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.8M sodium citrate, 0.1M Tris pH 8.5