8VIV

Crystal structure of FBF-2 RBD in complex with gld-1 FBEa* RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	98.569, 98.569, 107.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.270 - 2.200
R-factor	0.1992
Rwork	0.197
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.405
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmeas	0.160	1.240
Rpim	0.070	0.560
Number of reflections	29170	1511
<I/σ(I)>	13.5
Completeness [%]	99.4	99.8
Redundancy	5	4.7
CC(1/2)	0.990	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% PEG 20000, 2% (v/v) dioxane, 0.1 M bicine, pH 9.0