8VIU

The DNA 16-mer sequence 5'-GCTGCGTTAACGCAGC-3' bound by a DB1992 stacked dimer

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979340
Spacegroup name	H 3 2
Unit cell lengths	38.345, 38.345, 162.141
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.530 - 1.490
R-factor	0.2273
Rwork	0.225
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.747
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.530	1.543
High resolution limit [Å]	1.490	1.490
Rmerge	0.056	0.552
Rmeas	0.059	0.580
Rpim	0.018	0.175
Number of reflections	7906	775
<I/σ(I)>	22.13	4.18
Completeness [%]	99.3	99.87
Redundancy	10.8	10.9
CC(1/2)	0.999	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	295	10mM HEPES, pH=8.6, 600mM CaCl2, 41% PEG200 mixed 1:1 with 1mM DNA duplex