8VIU
The DNA 16-mer sequence 5'-GCTGCGTTAACGCAGC-3' bound by a DB1992 stacked dimer
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979340 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 38.345, 38.345, 162.141 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.530 - 1.490 |
| R-factor | 0.2273 |
| Rwork | 0.225 |
| R-free | 0.24920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.747 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.530 | 1.543 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.056 | 0.552 |
| Rmeas | 0.059 | 0.580 |
| Rpim | 0.018 | 0.175 |
| Number of reflections | 7906 | 775 |
| <I/σ(I)> | 22.13 | 4.18 |
| Completeness [%] | 99.3 | 99.87 |
| Redundancy | 10.8 | 10.9 |
| CC(1/2) | 0.999 | 0.947 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 295 | 10mM HEPES, pH=8.6, 600mM CaCl2, 41% PEG200 mixed 1:1 with 1mM DNA duplex |
