Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 90.744, 90.744, 122.713 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 78.590 - 1.930 |
R-factor | 0.16915 |
Rwork | 0.168 |
R-free | 0.19903 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8dhg |
RMSD bond length | 0.019 |
RMSD bond angle | 1.990 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 78.590 | 78.590 | 2.180 |
High resolution limit [Å] | 1.930 | 12.800 | 1.930 |
Rmerge | 0.065 | 0.017 | 0.501 |
Rmeas | 0.084 | 0.023 | 0.657 |
Number of reflections | 42552 | 167 | 12765 |
<I/σ(I)> | 11.1 | ||
Completeness [%] | 95.6 | ||
Redundancy | 2.2 | 2.1 | |
CC(1/2) | 0.997 | 0.999 | 0.729 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.8 | 293 | 0.10 M NaAcetat_pH4,8 2.30 M (NH4)2SO4 30 % Glycerol, 18% (w/v) PEG 4000, 0,1M Na3Citrate pH=5.75 |