8VGQ

CryoEM structure of GNE-1952-alkylated KRAS G12C in complex with engineered conformationally rigid Fab 2H11.4DS

This is a non-PDB format compatible entry.

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Detector	FEI FALCON IV (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.8
Software	cryoSPARC,PHENIX,EPU,ISOLDE,UCSF ChimeraX
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION