8VG9
Crystallographic structure of Ruminococcus champanellensis xylanase (RcXyn30A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS SIRIUS BEAMLINE MANACA |
| Synchrotron site | LNLS SIRIUS |
| Beamline | MANACA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9770 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.620, 55.019, 63.329 |
| Unit cell angles | 90.00, 95.16, 90.00 |
Refinement procedure
| Resolution | 44.050 - 1.370 |
| R-factor | 0.1412 |
| Rwork | 0.140 |
| R-free | 0.16400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.050 | 1.419 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.100 | 0.885 |
| Rmeas | 0.109 | 0.886 |
| Rpim | 0.042 | 0.361 |
| Number of reflections | 81497 | 8035 |
| <I/σ(I)> | 11.53 | |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 6.6 | 5.8 |
| CC(1/2) | 0.998 | 0.667 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 37.5% (v/v) precipitants agents (25% (v/v) MPD, 25% (w/v) PEG 1000 and 25% (w/v) PEG 3350), 0.1 M buffer solution (TRIS base/bicine) pH 8.5 and 0.09 M halogens |
