8VFH

Ternary DNA Polymerase Beta bound to DNA containing primer terminal FapydG base-paired with a dC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-06
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	50.800, 80.400, 55.500
Unit cell angles	90.00, 107.90, 90.00

Refinement procedure

Resolution	24.650 - 2.010
R-factor	0.1836
Rwork	0.177
R-free	0.24560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.041
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.050
High resolution limit [Å]	2.010	2.010
Number of reflections	24631	1355
<I/σ(I)>	11.2
Completeness [%]	99.6
Redundancy	4.1
CC(1/2)	0.990	0.401

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	14-22% PEG 3350 0.05 M Imidazole pH 8.0 0.35 M Sodium Acetate