8VFE

Binary DNA Polymerase Beta bound to DNA containing primer terminal FapydG base-paired with a T

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-04
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	53.680, 79.287, 54.958
Unit cell angles	90.00, 106.15, 90.00

Refinement procedure

Resolution	24.890 - 2.090
R-factor	0.2029
Rwork	0.200
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.258
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.140
High resolution limit [Å]	2.090	2.090
Number of reflections	36973	1306
<I/σ(I)>	16.8
Completeness [%]	99.9
Redundancy	4.3
CC(1/2)	0.999	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	14-22% PEG 3350 0.05 M Imidazole pH 8.0 0.35 M Sodium Acetate