8VFD

Ternary DNA Polymerase Beta bound to DNA containing template FapydG incoming TTP analog

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-16
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	53.770, 79.070, 54.366
Unit cell angles	90.00, 107.91, 90.00

Refinement procedure

Resolution	25.020 - 2.100
R-factor	0.2034
Rwork	0.200
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.110
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.020	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	32606	1233
<I/σ(I)>	18.9
Completeness [%]	99.5
Redundancy	4
CC(1/2)	0.999	0.428

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	14-22% PEG 3350 0.05 M Imidazole pH 8.0 0.35 M Sodium Acetate