8VFC

Binary DNA Polymerase Beta bound to DNA containing primer terminal T base-paired with FapydG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-07
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	54.064, 79.728, 55.683
Unit cell angles	90.00, 105.67, 90.00

Refinement procedure

Resolution	24.740 - 2.480
R-factor	0.2029
Rwork	0.197
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.139
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.540
High resolution limit [Å]	2.480	2.480
Number of reflections	14431	715
<I/σ(I)>	15.1
Completeness [%]	90.9
Redundancy	3.4
CC(1/2)	0.996	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	14-22% PEG 3350 0.05 M Imidazole pH 8.0 0.35 M Sodium Acetate