8VFB

Ternary DNA Polymerase Beta bound to DNA containing primer terminal dA base-paired with FapydG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-05
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	53.854, 79.307, 54.702
Unit cell angles	90.00, 108.88, 90.00

Refinement procedure

Resolution	25.480 - 2.640
R-factor	0.1987
Rwork	0.196
R-free	0.22750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.583
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.480	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	8173	647
<I/σ(I)>	3.1
Completeness [%]	100.0
Redundancy	4.3
CC(1/2)	0.592	0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	14-22% PEG 3350 0.05 M Imidazole pH 8.0 0.35 M Sodium Acetate