8VF8
Binary DNA Polymerase Beta bound to DNA containing primer terminal dC base-paired with FapydG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-19 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.133, 79.436, 55.023 |
| Unit cell angles | 90.00, 105.37, 90.00 |
Refinement procedure
| Resolution | 24.790 - 1.980 |
| R-factor | 0.1943 |
| Rwork | 0.192 |
| R-free | 0.22160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.227 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 32838 | 1336 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 95.0 | |
| Redundancy | 2.6 | |
| CC(1/2) | 0.989 | 0.466 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 14-22% PEG 3350 0.05 M Imidazole pH 8.0 0.35 M Sodium Acetate |
