8VF8

Binary DNA Polymerase Beta bound to DNA containing primer terminal dC base-paired with FapydG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-19
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	54.133, 79.436, 55.023
Unit cell angles	90.00, 105.37, 90.00

Refinement procedure

Resolution	24.790 - 1.980
R-factor	0.1943
Rwork	0.192
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.227
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.010
High resolution limit [Å]	1.980	1.980
Number of reflections	32838	1336
<I/σ(I)>	12.6
Completeness [%]	95.0
Redundancy	2.6
CC(1/2)	0.989	0.466

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	14-22% PEG 3350 0.05 M Imidazole pH 8.0 0.35 M Sodium Acetate