8VEX

Crystal structure of PRMT5:MEP50 in complex with MTA and oxamide compound 28

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97930
Spacegroup name	I 2 2 2
Unit cell lengths	99.470, 136.350, 177.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.200 - 2.790
R-factor	0.22861
Rwork	0.226
R-free	0.26972
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	1.437
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.200	2.940
High resolution limit [Å]	2.790	2.790
Rmerge	0.087	0.763
Rmeas	0.095	0.829
Rpim	0.037	0.320
Total number of observations		28685
Number of reflections	30399	4354
<I/σ(I)>	15.1	2.5
Completeness [%]	99.9
Redundancy	6.6	6.6
CC(1/2)	0.999	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	291	10% PEG 4000, 0.2M magnesium chloride, 0.1M sodium citrate (pH 6.0)