8VEW

Crystal structure of PRMT5:MEP50 in complex with MTA and oxamide compound 24

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	I 2 2 2
Unit cell lengths	100.560, 137.732, 177.676
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.650 - 2.690
R-factor	0.22293
Rwork	0.220
R-free	0.26949
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.004
RMSD bond angle	1.385
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.650	2.820
High resolution limit [Å]	2.690	2.690
Rmerge	0.150	1.326
Rmeas	0.156	1.380
Rpim	0.042	0.378
Total number of observations		59363
Number of reflections	34631	4539
<I/σ(I)>	15.1	2.4
Completeness [%]	100.0
Redundancy	13.5	13.1
CC(1/2)	0.999	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	291	10% PEG 4000, 0.2M magnesium chloride, 0.1M sodium citrate (pH 6.0)