8VET
Crystal structure of PRMT5:MEP50 in complex with MTA and oxamide compound 1
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-02 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.07812 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 103.190, 137.030, 178.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.700 - 2.630 |
R-factor | 0.22691 |
Rwork | 0.224 |
R-free | 0.27691 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.002 |
RMSD bond angle | 1.312 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.700 | 2.700 |
High resolution limit [Å] | 2.630 | 2.630 |
Rmerge | 0.088 | 1.451 |
Rmeas | 0.091 | 1.510 |
Rpim | 0.025 | 0.416 |
Total number of observations | 35949 | |
Number of reflections | 37944 | 2774 |
<I/σ(I)> | 19.6 | 1.9 |
Completeness [%] | 99.8 | |
Redundancy | 13.5 | 13 |
CC(1/2) | 0.999 | 0.894 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 291 | 10% PEG 4000, 0.2M magnesium chloride, 0.1M MES (pH 6.5) |