8VER
Structure of YicC endoribonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92141 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 112.923, 76.539, 136.345 |
| Unit cell angles | 90.00, 100.29, 90.00 |
Refinement procedure
| Resolution | 38.340 - 2.800 |
| R-factor | 0.2284 |
| Rwork | 0.226 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.378 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.449 | 3.146 |
| High resolution limit [Å] | 2.797 | 2.797 |
| Number of reflections | 37513 | 1876 |
| <I/σ(I)> | 8.8 | 1.5 |
| Completeness [%] | 93.0 | 62.6 |
| Redundancy | 4.6 | |
| CC(1/2) | 0.996 | 0.539 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.1 M Tris pH 8.5, 25% PEG 3350, 0.3 M Lithium Sulfate |
